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【Objective】 To investigate the detection of pathogenic microorganisms in umbilical cord blood and maternal blood from 2012 to 2021, so as to improve the collection of umbilical cord blood and guarantee the safety of umbilical cord blood hematopoietic stem cells (HSC) . 【Methods】 Detection results of pathogenic microorganisms of umbilical cord blood and maternal blood among 64 077 cases from Tianjin Cord Blood Bank from 2012 to 2021 were retrospectively analyzed. 【Results】 A total of, 2 072 cases (3.23%) were detected positive, among which, 184 cases (0.29%) were positive for aerobic bacteria culture, 1 504 cases (2.34%) were positive for anaerobic bacteria culture, and 384 cases (0.60%) were positive for both aerobic and anaerobic bacteria culture. From 2012 to 2021,the overall positive rate showed a downward trend, with a difference in the positive rate between each year (P<0.05). The positive rate of anaerobic bacteria was higher than that of aerobic bacteria and that of anaerobic and aerobic bacteria (P<0.05). After Gram staining, the microscopic detection rate of bacterial positive samples was highest in G- bacilli, followed by G+ bacilli, G+ cocci, G- cocci and others. Among the 64 077 cases, 169 cases (0.26%) showed reactivity in cord blood tests and 1 231 cases (1.92%) showed reactivity in maternal blood tests. Umbilical cord blood and maternal blood HIV-Ag/Ab tests showed reactivity after initial screening. After confirmation by Western blotting, there was 1 case of uncertain maternal blood, while the rest were negative. The reactive rates of anti-TP (0.12%) and anti- HCV (0.11%) in umbilical cord blood were higher than those of HBsAg (0.03%) and CMV-IgM (1/64 077).There was a difference in the reactive rate of anti-TP detection in umbilical cord blood between different years (P<0.05),while there was no statistically significant difference in that of HBsAg, anti-HCV and CMV-IgM (P> 0.05).The reactive rate of HBsAg in maternal blood (1.38%) was higher than that of CMV-IgM(0.29%) , anti-TP(0.13%) and anti-HCV (0.12%) . There were differences in the reactive rates of HBsAg, anti-HCV ,and anti-TP in maternal blood among different years (P<0.05),and that of HBsAg showed a decreasing trend, while the reactive rate of CMV-IgM was not statistically significant (P>0.05). The reactive rates of HBsAg and CMV-IgM detected in maternal blood were significantly higher than those in umbilical cord blood (P<0.05) . The reactive rates of anti-HCV and anti-TP in maternal blood were consistent with those in umbilical cord blood (P>0.05). 【Conclusion】 The reactive rates of anti-HIV and CMV-IgM in cord blood, and that of anti-HIV in maternal blood are low, but those of anti-TP and anti-HCV in cord blood are relatively high. The reactive rate of HBsAg is high in maternal blood,but with a downward trend,but low in umbilical cord blood due to maternal-infantile transmission blocking. The detection of transfusion transmitted pathogens and bacteria plays a critical role on the safety of umbilical cord blood HSCs. Effective detection of transfusion transmitted pathogens and culture of bacteria are the key to ensure the quality of umbilical cord blood, which can improve the safety of umbilical cord blood HSCs transplantation.
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Objective]The clinical efficacy of TCM directed through drug treatment of pain for the first time with the temperature needle shoulder-hand syndrome after caused by stroke.[Methods]The 90 cases were randomly divided into treatment and control groups.The treatment group was directed through medicine with traditional Chinese medicine acupuncture treatment temperature ,the control group using only warm acupuncture.Respectively, before treatment, the first 30 days of oral grading score(VRS) was assessed for limb pain.[Results]After treatment ,the patient's limb pain scores were significantly lower than before treatment,treatment group decreased more significantly than the control group,the difference was statistically significant(P﹤0.05). [Conclusion]TCM directed through drug treatment and needle with warm warm acupuncture alone can significantly ease the pain of limb ,but TCM directed through drug treatment is better than warm needle acupuncture alone.
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Objective To investigate the effect of obstructive sleep apnea syndrome (OSAS) on coronary atherosclerosis plaque.Methods A total of 126 old patients with coronary artery disease (CAD) were selected and classified into two groups according to their results of polysomnography and coronary angiography:OSAS plus CAD group (n= 62);no OSAS group (n=64).Peripheral venous blood samples were taken,and the levels of total cholesterol (TC),triglyceride (TG),high-density lipoprotein (HDL-C),low-density lipoprotein (LDL-C),white blood cell counts,neutrophil percent and high sensitive C-reactive protein (hs-CRP) were measured.The cases with coronary lesion,Gensini scores and atherosclerosis plaque were recorded according to coronary arteriography and 64- slice spiral coronary CT angiography.Results The levels of TC,TG and LDL-C were higher in OSAS plus CAD group than in no OSAS group [(5.76+0.85) mmol/L vs.(4.26+0.78) mmol/L,(3.37+1.97) mmol/L vs.(1.72±0.54) mmol/L,(3.65±0.58) mmol/L vs.(2.91±0.58) mmol/L,t= 2.959,3.556,2.165,all P<0.05].The levels of HDL were lower in OSAS plus CAD group than in no OSAS group [(0.86±0.20) mmol/L vs.(1.13±0.14) mmol/L,t=2.545,P<0.05].The white blood cell counts,neutrophil percent and serum hs-CRP levels were higher in OSAS plus CAD group than in no OSAS group (all P<0.05).In OSAS plus CAD group,the prevalence of multivessel disease was 51%,Gensini scores were (23.6±20.7) and coronary atherosclerosis plaques were (67.6 ±9.7),while the prevalence of multivessel disease was 30 %,Gensini scores were (18.9±19.4) and coronary atherosclerosis plaques were (39.3 ± 9.4) in no OSAS group.There were significant differences in the three parameters between the two groups (x2 or t=5.39,2.048,19.001,all P<0.05).Conclusions OSAS is associated with coronary artery disease and can accelerate the formation of coronary atherosclerosis plaque.
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BACKGROUND:Multiple studies have shown that obstructive sleep apnea is correlated with cardiovascular diseases,and plays an important role in occurrence and development of cardiovascular diseases.Nevertheless,we have little knowledge about effects of obstructive sleep apnea on results and prognosis of coronary artery interventional therapy.OBJECTIVE:To investigate the effect of obstructive sleep apnea on restenosis in patients undergoing successful coronary artery stent implantation.METHODS:A total of 76 patients with acute coronary syndrome (ACS) were selected,including 59 males and 17 females,with an average age of (67±10) years,at the Department of Cardiology,Xingtai Municipal People's Hospital from January 2005 to December 2008.There were 45 patients with acute myocardial infarction and 31 patients with unstable angina pectoris,All patients were subjected to coronary artery stent implantation,coronary angiography.The coronary lesion cases and Gensini grades were recorded.Of them,63 patients ceceived coronary artery interventional therapy.All patients underwent polysomnography.The results of quantitative coronary angiography were analyzed in the course of operation and at 6-months following-up.RESULTS AND CONCLUSION:A total of 37 of the 76 patients had obstructive sleep apnea.The prevalence of multivessel disease was 51% and Gensini grades was 23.6±20.7,which was higher than patients without obstructive sleep apnea (P=0.004).There were no significant differences between the two groups for median reference vascular diameter,median lesion length before operation,acute gain besides minimum lumen diameter after operation (P＞0.05).The rate of coronary artery in stent restenosis in patients with obstructive sleep apnea was 37%,which was higher than patients without obstructive sleep apnea over a 6-month follow-up (P=0.021).
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BACKGROUND:Stent restenosis is a pathological process characterized by inflammatory healing response. Inflammatory cells play an important role in stent restenosis,thus,it can be controlled to reduce stent restenosis. OBJECTIVE:To observe the changes of high-sensitivity C-reactive protein (hs-CRP) and complement C3 before and after coronary stent implantation in patients with acute coronary syndrome,and to study the relation of inflammatory reactions and stent restenosis. DESIGN,TIME AND SETTING:A contrast observation was performed at the Department of Cardiology,People's Hospital of Xingtai between December 2005 and May 2009. PARTICIPANTS:A total of 65 patients with acute coronary syndrome and treated by percutaneous transluminal coronary intervention were collected. METHODS:According to coronary anglography,sirolimus-eluting stent (Firebird stent) was selected and implanted to cure acute coronary syndrome. MAIN OUTCOME MEASURES:Blood routine examination was performed before stent implantation and coronary anglography were used over a 6-month follow-up. The hs-CRP and complement C3 concentration was detected prior to,48 hours and 6 months after operation. RESULTS:Fifty-six patients were followed up,and the restenosis rate was 9% (5/56). The levels of hs-CRP,complement C3 in peripheral vein blood was obviously higher at 48 hours after implantation than prior to operation (P
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Objective To investigate the myocardium protective effect of immunoinflammatory response induced by preinfarction angina. Methods Eighty-eight patients of acute myocardial infarction were divided into preinfarction angina group (48 subjects) and sudden onset group (40 subjects). The incidence of severe arrhythmia,heart failure,cardiac shock and in-hospital mortality were assessed in the two groups. The myocardial infarction size,ventricular function,coronary angiography were compared between the two groups. Some immunoinflammatory markers levels were detected. Results In preinfarction angina group,the incidences of severe arrhythmia,heart failure,and in-hospital mortality were lower (P
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BACKGROUND: Borneol can open blood-brain barrier (BBB) but the mechanisms are not very clear. Histamine and 5-hydroxtryptamine can take part in regulation of permeability of BBB. There is not report on the rela tion between the effect of opening BBB of borneol and the regulation of permeability of BBB of histamine (HA)/5-hydroxytryptamine (5-HT). OBJECTIVE: To study the relation between the effect of opening BBB of bornool and the regulation of permeability of BBB of histamine (HA)/5-hydroxytryptamine (5-HT).DESIGN: A completely randomized and controlled study.SETTING: Clinical Pharmacological Institute of University of Traditional Chinese Medicine of Guangzhou.MATERIALS: The experiment was carried out at the Clinical Pharmacological Institute of University of Traditional Chinese Medicine of Guangzhou from September 2003 to February 2004. Totally 104 healthy male SD rats, weighting 230-27.0 g, supplied by Guangdong Medical Experimental Animal Center, were selected.METHODS: The rats were randomly divided into 13 groups: pre-medicine group and post-medicine group (high, moderate and low dosage group and 5, 20, 45 and 60 minutes group in each dosage group) with 8 in each group. Borneol was mixed as 10% millet oil suspension. Rats were fasted before experiment for whole night, and medicine was perfused on the next morning with the high, moderate and low dosage of 0.15, 0.12 and 0.09 g/kg respectively. Hypothalami of rats was selected at various time points to make biological samples. Contents of HA and 5-HT were assayed with HPLC system electrochemical detector.MAIN OUTCOME MEASURES: Changes of level of histamin (HA) and 5-HT in hypothalamus of rats after administration of borneol.RESULTS: Data of totally 104 rats was entered the final analysis. ① Contents of HA and 5-HT in hypothalami were 2.07±0.54 μg/g and 1.45±0.14 μg/g respectively. ② The level of HA in hypothalamus of rats after different doses of borneol were higher than that of before administration. Comparing the level of HA in 20 minutes after moderate dose with before administration, the level of HA in 20 minutes was increased significantly [(3.36±0.21) μg/g, P < 0.01], the others of moderate dose, the 45 minutes after high dose, the 20 and 45 minutes after low dose were also increased significantly than before administration (P < 0.05). ③ After administration of different doses of borneol, the level of 5-HT after high dose were higher than that of before administration [5, 20, 45 and 60 minutes after medicine: (1.90±0.32), (3.28 ±0.25), (2.66±0.46), (2.80±0.34) μg/g, respectively; (P < 0.05 or P < 0.01)]; the level of 5-HT after 5, 20 and 45 minutes of moderate and low dose were increased significantly too (P < 0.05 or P < 0.01).CONCLUSION: Borneol could open BBB by increasing levels of HA and 5-HT in hypothalamus of rats.Borneol mediates opening of BBB by increasing levels of HA and5-HT in rats.
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Objective To investigate the prevalence rate of hyperlipidemia in the middle-aged and elderly in Guangxi Hei Yi Zhuang population. Methods A total of 657 people of Hei Yi Zhuang nationality aged 40 years and over were surveyed. Blood pressure, body height, body weight, serum lipid and apolipoprotein levels were measured, and both body surface areas and body mass index were calculated. The results were compared with those in 520 people of Han nationality who also live in that district. Results The prevalence of hypercholesterolemia, hypertriglyceridemia, and hyperlipidemia in Hei Yi Zhuang vs. Han population were 28.9% vs. 35.8%(P0.05), and 36.2% vs. 42.3%(P0.05) respectively. Conclusions Prevalence of hyperlipidemia of Hei Yi Zhuang is lower than that of Han ethnic group, which might results from different dietary habit, life style, physical activity, and even genetic background.
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Objective To establish a method for the determination of arctiin in Pifu Zhiyang Tablets by SPE-HPLC . Methods SPE-HPLC method was performed on a Kromasil C18 column(150 mm?4.6 mm ,5 ?m),the mobile phase consisted of methanol -1 %acetic acid (35 ∶65) ,the wavelength was 283nm . Results The linear range of arctiin was 1.5～4.5 ?g(r= 0. 999 8) ,the average recovery was 98.18 %with RSD of 2.08 %. Conclusion This method is accurate ,reliable and reproducible. It can be used for the determination of arctiin in Pifu Zhiyang Tablets .